Ball, David; Peccoud, Jean (2021). CIL:39563, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WW7HR6

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged MCM1.

MCM1 is a transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes

These phase,gfp z-series images are part of an image group that ranges from CIL:39561-39565. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

• 2021

• Ball, David
• Peccoud, Jean

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions

• Cell Image Library Group ID: 10628

• Saccharomyces cerevisiae

• MCM1 protein
• Nuclear chromatin
• Nucleus

• Arginine metabolic process
• Cell cycle
• DNA bending activity
• DNA binding
• DNA replication origin binding
• DNA-dependent DNA replication initiation
• Regulation of transcription from RNA polymerase II promoter
• Regulation of transcription, DNA-dependent
• Sequence-specific DNA binding transcription factor activity
• Transcription, DNA-dependent

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39563

Doi: https://doi.org/10.6075/J0WW7HR6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39563

Reference

• Ball DA, Marchand J, Poulet M, Baumann WT, Chen KC, Tyson JJ, Peccoud J. Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry. PLoS One. 2011;6(10):e26272. https://doi.org/10.1371/journal.pone.0026272
• PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26. https://www.ncbi.nlm.nih.gov/pubmed/?term=22046265

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2022-08-12