Allen, Richard (2021). CIL:12978, Paramecium multimicronucleatum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02F7MHC

Four parasomal sacs showing them in longitudinal section each at the base of a surface depression. Trichocysts extend into each lateral ridge (lateral ridges do not have kinetodesmal fibers). Bulbous ends of parasomal sacs are covered with clathrin cages. TEM taken on 4/7/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12611) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 3920

• Paramecium multimicronucleatum

• Axoneme
• Cell cortex
• Ciliated Protist
• Coated pit
• Eukaryotic cell
• Eukaryotic Protist
• Plasma membrane
• Trichocyst

• Cilium axoneme assembly
• Clathrin coat assembly
• Cortical cytoskeleton organization
• Trichocyst localization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12978

Doi: https://doi.org/10.6075/J02F7MHC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12978

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12