Allen, Richard (2021). CIL:7338, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TH8KQJ

High resolution transmission electron micrograph of a portion of the macronucleus of Opercularia coarctata containing irregularly shaped heterochromatin bodies and elliptical nucleoli. Ribosome-like precursors can be seen at the margins of the nucleoli.

• 2021

• Allen, Richard

TEM taken on 6/11/69 by R. Allen with Philips 300 operating at 60kV. Neg. 23,200X. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Opercularia
• Opercularia coarctata

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Heterochromatin
• Macronucleus
• Nuclear envelope
• Nuclear pore
• Nucleolus

• Cambridge Culture Collection, Cambridge, England, # LB1657/1
• Chromatin organization
• Nucleolus organization
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7338

Doi: https://doi.org/10.6075/J0TH8KQJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7338

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12