Shroff, Hari; Galbraith, Catherine G.; Galbraith, James A.; White, Helen; Gillette, Jennifer; Olenych, Scott; Davidson, Michael W.; Betzig, Eric (2021). CIL:38598, Homo sapiens, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05Q4TRJ

Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-alpha-actinin (red) and tdEos-vinculin (green). This image reveals that although conventional diffraction limited microscopy indicates alpha-actinin and vinculin colocalize in focal adhesions, PALM clearly shows that they these molecules are spatially segregated. The DIC corresponds to the same image field is CIL 38597 and the diffraction limited TIRF image is CIL 38599. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13.

• 2021

• Betzig, Eric
• Davidson, Michael W.
• Galbraith, Catherine G.
• Galbraith, James A.
• Gillette, Jennifer
• Olenych, Scott
• Shroff, Hari
• White, Helen

Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: portrayed image
Imaging mode: PALM (photoactivation localization microscopy)
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Dronpa; tdEos
Processing history: probability density map

• Cell Image Library Group ID: 9553

• Homo sapiens

• Alpha actinin
• Cardiac muscle cell
• Early embryonic cell
• Striated muscle dense body
• Z-line component

• Focal adhesion assembly
• Foreskin fibroblast
• Molecular organization of focal adhesion components

• data
• image

• No linguistic content; Not applicable

Samplenumber: 38598

Doi: https://doi.org/10.6075/J05Q4TRJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38598

Reference

• Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. Hari Shroff, Catherine G. Galbraith, James A. Galbraith, Helen White, Jennifer Gillette, Scott Olenych, Michael W. Davidson, and Eric Betzig. December 18, 2007, 104 (51) 20308-20313. https://doi.org/10.1073/pnas.0710517105
• Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220. https://www.ncbi.nlm.nih.gov/pubmed/?term=18077327

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2022-08-12