Ip, Wallace (2021). CIL:2874, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZK5FQP

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in 0.2% saponin. The fibers were quick-frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:1454.

• 2021

• Ip, Wallace

Preparation: glutaraldehyde fixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1932

• Oryctolagus cuniculus

• Cytoskeleton
• Skeletal muscle cell

• ATPase activity, coupled to movement of substances
• Skeletal muscle contraction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 2874

Doi: https://doi.org/10.6075/J0ZK5FQP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL2874

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12