CIL:35128, Mus musculus, macrophage
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- Collection
- Cite This Work
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Cammer, Michael; Cox, Dianne (2021). CIL:35128, Mus musculus, macrophage. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZC81T4
- Description
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Primary bone marrow macrophages derived from mouse plated on a coverslip and imaged live. Internal Reflection Microscopy (IRM) shows direct apposition of the cells to coverslip with very little depth of field. Note the light gray uniform background extends under the cells' footprints demonstrating that the cells do not adhere tightly to the substrate. At the center of the image is a large polarized lamellipod of a crawling cell. The small spot below the cell is the tip of glass micropipette delivering CSF (colony stimulating factor) locally and the cell may be responding.
- Date Issued
- 2021
- Researchers
- Methods
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Live bone marrow macrophages plated in MatTek dish and imaged with a 60X N.A. 1.4 phase 3 Olympus objective on an IX71 microscope with a Cooke Sensicam QE camera and IPLab software running on a Dell Windows XP computer. The reflection microscopy was performed by removing the emission filter from a standard narrow pass rhodamine filter cube. Light was at approximately 570 nm and reflection leaked through the dichroic to allow for imaging.
- Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: interference reflection contrast (IRM)
Parameter imaged: interference reflection contrast (IRM)
Source of contrast: interference contrast
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 35128
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35128
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12