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CIL:13757, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell

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Cite This Work

Hartman, Tiffiney R.; Zinshteyn, Daniel; Schofield, Heather K.; Nicolas, Emmanuelle; Okada, Ami; O'Reilly, Alana M. (2021). CIL:13757, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FJ2FHV

Description

The Hedgehog (Hh) pathway is activated in follicle stem cells of animals lacking boi (boi[e]; this image), relative to wild-type Drosophila (CIL# 13756), based on activated cubitus interruptus (ci) staining (green). Follicle cells are highlighted with anti-Fas-3 (red) and germ cells are highlighted with anti-vasa (blue). Image is Supplemental Figure S3D in J Cell Biol. 2010. 191: 943-952.

Date Issued
  • 2021
Researchers
Methods

Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). Primary antibodies were 1:2,000 rabbit anti-Vasa (Hay et al., 1990); 1:25 mouse anti-Fas3 (Developmental Studies Hybridoma Bank [DSHB] and anti-activated ci. Secondary antibodies used were FITC, Cy3, and Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).

Technical Details

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; Fluorescein (FITC); Cy3; Cy5
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

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Identifier

Samplenumber: 13757

Doi: https://doi.org/10.6075/J0FJ2FHV

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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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  • UC Regents
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

Last Modified

2022-08-12