Allen, Richard (2021). CIL:36644, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0416W02

Large patches of rough ER surrounded by Golgi stacks (visible only at high magnification) have formed in the cytosol along with fields of lipid bodies. By day 2 these ER and lipid fields will be dispersed or depleted. For culture age study see Fok and Allen, Eur. J. Cell Biol. 25:182-193, 1981. TEM taken on 4/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 4,050X. Bar = 1µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Endoplasmic reticulum
• Eukaryotic cell
• Eukaryotic Protist
• Golgi stack

• Endoplasmic reticulum organization
• Golgi organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36644

Doi: https://doi.org/10.6075/J0416W02

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36644

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2022-08-12