CIL:37934, Caenorhabditis elegans, early embryonic cell
CIL source metadata (JSON)
File Size |
|
File Format |
|
- Collection
- Cite This Work
-
Goehring, Nathan W.; Hoege, Carsten; Grill, Stephan W.; Hyman, Anthony A. (2021). CIL:37934, Caenorhabditis elegans, early embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HM573F
- Description
-
Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug latrunculin A (disrupts the actin cytoskeleton) at frame 13. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations.
- Date Issued
- 2021
- Researchers
- Methods
-
C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with latrunculin A. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video2 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.
- Technical Details
-
Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; time lapse microscopy
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: mCherryFP; EGFP; Hoechst
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
- Topics
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Identifier
-
Samplenumber: 37934
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37934
- J Cell Biol. 2011. 193(3): 583-594. https://www.ncbi.nlm.nih.gov/pubmed/?term=21518794
- BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/
Source data
Reference
Other resource
- License
-
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12