Uetake, Yumi; Sluder, Greenfield (2021). CIL:35201, Homo sapiens, retinal pigment epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RB73HB

Movie showing motility and morphology of cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) arrested in G1 using 0.08 uM nocodazole (Uetake and Sluder 2010, Curr Biol 20:1666-1671).
The time series used to prepare the movie is grouped in the Cell Image Library.

• 2021

• Sluder, Greenfield
• Uetake, Yumi

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using HC Image 2.0.1.11 software.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8518

• Homo sapiens

• Retinal pigment epithelium

• Cell morphology
• Cell motility
• HTERT-RPE1

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 35201

Doi: https://doi.org/10.6075/J0RB73HB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35201

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12