Allen, Richard (2021). CIL:9832, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WH2P5Z

A view of cross-sectioned rows of basal bodies in Nassula exposing the absence of cilia. Only a few basal bodies may have attached cilia as seen by the few cilia present in the upper right row. Basal bodies extend to the tips of the cytoplasmic mounds and each mound has a parasomal sac associated to one side. Unlike many other ciliates, the cartwheel occupies most of the basal body lumen. TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7747) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3478

• Nassula

• Absence of cilia
• Cell by organism
• Cell cortex
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body

• Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
• Ciliary or flagellar motility
• Cortical cytoskeleton organization
• Microtubule cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9832

Doi: https://doi.org/10.6075/J0WH2P5Z

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9832

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12