Sage, M.; Allen, Richard (2021). CIL:12302, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09G5KZJ

Lamellae of cytopharynx in the opisthe of a dividing cell. This cytopharynx is not yet functional and lacks most of the medium dense vesicles that accumulate between the lamellae. Toxicysts are present. One toxicyst has myelin-like layers at the level it was sectioned. Bacteria align adjacent to the cell. they may be symbiotic. TEM taken on 5/9/69 by M. Sage with Philips 300 operating at 60kV. Neg. 9,250X. Bar = 1µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A high resolution image is in the library (CIL:38864) and is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard
• Sage, M.

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 9814

• Didinium nasutum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cortical microtubule cytoskeleton
• Cytopharynx
• Eukaryotic cell
• Eukaryotic Protist
• Extrusomes

• Carolina Biological Supply Company, NC, U.S.A.
• Cortical cytoskeleton organization
• Cortical microtubule organization
• Cytopharyngeal microtubules
• Plasma membrane organization
• Structural constituent of cytoskeleton
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12302

Doi: https://doi.org/10.6075/J09G5KZJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12302

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12