Allen, Richard (2021). CIL:7340, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PR7TZC

A high resolution overview of Opercularia coarctata showing that cytostome divides the peristome from the cytopharynx. The cytopharynx starts where the alveoli end in the oral region. TEM taken on 6/13/69 by R. Allen with Philips 300 operating at 60kV. Neg. 5,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is suitable for quantitative analysis.

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3571

• Opercularia
• Opercularia coarctata

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cytoplasm
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus
• Oral apparatus

• Cambridge Culture Collection, Cambridge, England, # LB1657/1
• Cortical cytoskeleton organization
• Cytoplasm organization
• Oral organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7340

Doi: https://doi.org/10.6075/J0PR7TZC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7340

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2022-08-12