Apaja, Pirjo M.; Xu, Haijin; Lukacs, Gergely L. (2021). CIL:13699, Chlorocebus aethiops. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C8280P

This is one of a group of four images in Figure S5 in Apaja et al., JCB 2010, that support the conclusion that the WT vasopressin type2 receptor (V2R) and the V2R W164S mutant (that causes nephrogenic diabetes insipidus) have different post-endocytic targeting. The sorting of endocytosed myc-tagged WT and W164S V2R was determined by indirect immunolabeling of internalized receptors in transiently transfected COS7 cells. The WT and temperature (26 degrees C) rescued mutant receptors were labelled with anti-myc Ab in live cells (1h) and then chased in Ab-free medium (1h, 37 degrees C) before indirect immunolabeling. Endosomes were labeled with 10 µg/ml Alexa Fluor 594–transferrin uptake for 1 h at 37°C. Fluorescence micrographs were obtained by a microscope (LSM510 or LSM710; Carl Zeiss, Inc.) equipped with a Plan-Apochromat 63×/NA 1.4 objective in multitrack mode. This single optical section shows that the internalized V2R W164S mutant is not found in endosomes, the normal location of endocytosed WT V2R. Companion images show that the mutant V2R, however, is found in lysosomes.

• 2021

• Apaja, Pirjo M.
• Lukacs, Gergely L.
• Xu, Haijin

Preparation: crosslinking-fixative fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; Alexa Fluor 594
Data qualification: Processed

• Cell Image Library Group ID: 7836

• Chlorocebus aethiops

• Early endosome
• Plasma membrane

• COS-7
• G-protein coupled receptor internalization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13699

Doi: https://doi.org/10.6075/J0C8280P

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13699

Reference

• JCB 2010, 191:553-570 https://www.ncbi.nlm.nih.gov/pubmed/?term=20974815

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2022-08-12