Allen, Richard (2021). CIL:7335, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08W3CB2

High resolution micrograph showing an overview of the peristome of Opercularia coarctata. The corrugated wall, the equivalent to the ribbed wall of Paramecium, contains tubular projections of alveoli and cross sections of two ribbons of microtubules, one of 2 microtubules and one of 4 microtubules. The ends of these ribbons, at their origins, are covered by a myoneme which, in turn, is accompanied by a layer of ER. The microtubules will extend along the wall of the peristome and some will pass beyond the cytostome to form the lamellae of the cytopharynx. Vesicles bind to the lamellae and provide membrane for the nascent food vacuole. Oral polykinetids, myoneme, endoplasmic reticulum, contractile vacuole, microtubular ribbons, pellicular pores, and Golgi complex can be seen. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is suitable for quantitative analysis

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3591

• Opercularia
• Opercularia coarctata

• Cell by organism
• Ciliated Protist
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Oral apparatus

• Cambridge Culture Collection, Cambridge, England, # LB1657/1
• Ingestion
• Intracellular organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7335

Doi: https://doi.org/10.6075/J08W3CB2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7335

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12