Allen, Richard; Schroeder, C. (2021). CIL:36710, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0V69H8G

Details of the binding of the carrier vesicle to a microtubule. Four bridges, thought to be cytoplasmic dynein arms, span the 40nm space between these two structures. The membrane of the vesicle has been fractured to expose its smooth E-face. TEM taken on 5/18/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.1µm. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:40507) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/). Published in J. Cell Biol. 111:2553-2562, 1990. Adapted with permission.

• 2021

• Allen, Richard
• Schroeder, C.

Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed

• Cell Image Library Group ID: 10997

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Cytoplasmic dynein complex
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule associated complex
• Transport vesicle

• Cytoplasmic microtubule organization
• Vesicle docking involved in exocytosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36710

Doi: https://doi.org/10.6075/J0V69H8G

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36710

Reference

• J Cell Biol. 1990 Dec;111(6 Pt 1):2553-62. https://www.ncbi.nlm.nih.gov/pubmed/?term=2148940

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2022-08-12