Hartman, Tiffiney R.; Zinshteyn, Daniel; Schofield, Heather K.; Nicolas, Emmanuelle; Okada, Ami; O'Reilly, Alana M. (2021). CIL:13752, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WQ02JR

Expression of UAS-tauGFP (the tau-GFP fusion protein binds microtubules; green) under control of 109-30-Gal4 in wild-type Drosophila germaria. 109-30-Gal4 drives UAS-tauGFP expression in follicle stem cells and their progeny cells (see also CIL# 13753). Nuclei are in red. Image correlates to Supplemental Figure S2B in J Cell Biol. 2010. 191: 943-952.

• 2021

• Hartman, Tiffiney R.
• Nicolas, Emmanuelle
• O'Reilly, Alana M.
• Okada, Ami
• Schofield, Heather K.
• Zinshteyn, Daniel

Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). Primary antibody was anti-GFP. Secondary antibodies used were either FITC, Cy3, or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). For nuclear staining, fixed ovaries were incubated for 15 min with Draq5 (Cell Signaling Technology). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: primary antibody plus labeled secondary antibody; membrane-permeant probe; EGFP; DRAQ5
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8382

• Drosophila melanogaster

• Follicle cell
• Follicle stem cell
• Germ line cell
• Germ line stem cell

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13752

Doi: https://doi.org/10.6075/J0WQ02JR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13752

Reference

• J Cell Biol. 2010. 191: 943-952. https://www.ncbi.nlm.nih.gov/pubmed/?term=21098113

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12