Ammar, El-Desouky; Hogenhout, Saskia A. (2021). CIL:12591, Spiroplasma kunkelii, Zea mays, plant cell, prokaryotic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09W0D6V

Micrograph of a semi-thick (1-2 µm) section of an infected maize leaf, with silver-enhanced immunogold labeling, that shows dark staining of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates in phloem tissue. The section was counter stained with toluidine blue. CIL:19129, from the same image group, is a labeled version of this. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. dditional related images from this contributor are available in the Library.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.

Preparation: tissue in epoxy resin embedment; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: bright-field microscopy
Imaging mode: widefield illumination
Parameter imaged: absorption of illumination
Source of contrast: differences in adsorption or binding of stain
Visualization methods: toluidine blue; gold
Data qualification: Processed

• Cell Image Library Group ID: 9977

• Spiroplasma kunkelii
• Zea mays

• Plant cell
• Plant-type cell wall
• Prokaryotic cell

• Pathogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12591

Doi: https://doi.org/10.6075/J09W0D6V

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12591

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2022-08-12