Woodcock, Christopher (2021). CIL:700, Necturus maculosus, nucleate erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0251HDR

X-ray scattering shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contain more ordered chromatin than most nuclei. Here, fibers isolated from these nuclei have been negatively stained with aurothioglucose and imaged by TEM. ~30 nm chromatin fibers are seen, some exhibiting short regions with apparent structural order.

• 2021

• Woodcock, Christopher

Chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films, negatively stained with aurothioglucose and observed at 80Kv using a CM10 TEM.
See Woodcock CL et al. 1991. Ultrastructure of chromatin. I. Negative staining of isolated fibers. J Cell Sci. 99:99-106.

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: other negative stain
Processing history: raw unprocessed data

• Necturus maculosus

• Nuclear chromatin
• Nucleate erythrocyte

• Chromatin assembly
• Chromosome organization
• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 700

Doi: https://doi.org/10.6075/J0251HDR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL700

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2022-08-12