Howard, Louisa (2021). CIL:39790, Lytechinus pictus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HX1C1S

Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] gastrula. This is a higher magnification image of CIL39765. The embryo was split open to reveal a nice cross-section through the outer epithelial layer.This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.

• 2021

• Howard, Louisa

Images taken on a JEOL 35C SEM.
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold; visualization of contiguous regions; osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10724

• Lytechinus pictus

• Cell surface
• Epithelial cell

• Embryonic morphogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39790

Doi: https://doi.org/10.6075/J0HX1C1S

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39790

Other resource

• Dartmouth College Electron Microscope Facility (search on the string "sea urchin"): https://www.dartmouth.edu/emlab/gallery/index.php

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12