Hu, Chi-Kuo (2021). CIL:7240, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0222SSB

HeLa cell was fixed by MeOH and stained with microtubules (green) and central spindlin protein MKLP1 (red). MKLP1 (mitotic kinesin-like protein) was well aligned on midzone microtubules during cytokinesis.
Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against MLKP1 and FITC labeled DM1a antibody against microtubules. Secondary antibody was Alexa 594 for MLKP1. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected wti an OrCA ER CCD camera.
Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52.

• 2021

• Hu, Chi-Kuo

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; labeled primary antibody; Fluorescein (FITC); Alexa Fluor 594
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1455

• Homo sapiens

• Epithelial cell
• Microtubule

• HeLa
• Microtubule-based movement

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7240

Doi: https://doi.org/10.6075/J0222SSB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7240

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2022-08-12