CIL:40505, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Cite This Work
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Allen, Richard; Schroeder, C. (2021). CIL:40505, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0610Z6X
- Description
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High magnification view of a crushed mitochondrion showing the zipper-like row of 9nm diameter particles on the outer curve of the cristae and the single row of larger particles on the inner curve. There is an average of 1500 particles per square micrometer of 50-nm tubular cristae surface on the outer curve. These particles are thought to be the F1 units of the F1F0 ATP synthases of the mitochondria. The larger particles on the inner curve resemble complex I of the respiratory electron transport chain in size and relative abundance when compared to their relative quantities estimated from biochemical studies in other cells such as beef heart. This shows the highly ordered arrangement of some of the molecules of the oxidative phosphorylation and electron transport systems in Paramecium. TEM taken on 5/6//88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 63,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/). Published in J. Cell Biol. 108:2233-2240, 1989. Adapted with permission.
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film
Data qualification: Raw;spatialmeasurements - Series
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40505
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40505
- Allen RD, Schroeder CC, Fok AK. An investigation of mitochondrial inner membranes by rapid-freeze deep-etch techniques. J Cell Biol. 1989 Jun;108(6):2233-40. https://doi.org/10.1083/jcb.108.6.2233
- J Cell Biol. 1989 Jun;108(6):2233-40. https://www.ncbi.nlm.nih.gov/pubmed/?term=2525561
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12