CIL:39174, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:39174, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N29W97
- Description
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Detail of the origin of the cytopharyngeal microtubules along the left side of the cytopharynx that arise within the filamentous reticulum in contact with its electron-opaque nodes. This system does not arise from the basal bodies of the quadrulus although microtubules do arise along the dorsal edge of the basal bodies of this membranelle and curve between the ends of the cytopharyngeal ribbons and the alveoli of the buccal cavity. The end of the alveoli marks the transition of the buccal cavity into the cytopharynx. As shown on the left of this figure the cytopharyngeal microtubules curve over the cytostomal cord. From here the ribbons fan out into the cytosol. They bind to discoidal vesicles, acidosomes and carrier vesicles and move these vesicles toward the cytopharynx. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 37,500X.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 39174
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39174
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12