Allen, Richard (2021). CIL:9253, Coleps hirtus, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0B56HVZ

A high resolution of some tubules near a contractile vacuole that appear to be decorated with pegs similar to the spongiome of the CV of Paramecium. In Paramecium these pegs are known to be V-type proton pumps (V-ATPases). Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/2/69 by R. Allen with Philips 300. Neg. 12,500X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is available for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: film; unprocessed raw data

• Cell Image Library Group ID: 2156

• Coleps hirtus

• Ciliated Protist
• Contractile vacuolar membrane
• Contractile vacuole
• Cortical membranes
• Eukaryotic Protist
• Spongiome

• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Ionic regulation
• Multiple membrane interactions
• Response to water
• Water transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9253

Doi: https://doi.org/10.6075/J0B56HVZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9253

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2022-08-12