Sage, M.; Allen, Richard (2021). CIL:12299, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZK5FT1

A dividing Didinium nasutum. This large ciliate is capable of engulfing Paramecium. It has a thick ectoplasm containing numerous mucocysts embedded in its extensive epiplasm. Its external surface is coated with a layer of bacteria in this dividing cell. The cytoplasm is relatively compact under the conditions of fixation used. Electron opaque vacuoles are scattered in the cytoplasm. (For a more detailed report see Wessenberg and Antipa, Protistologica 4:427-447, 1968). TEM taken on 5/9/69 by M. Sage with Philips 300 operating at 60kV. Neg. 3,120X. Bar = 2µm.

• 2021

• Allen, Richard
• Sage, M.

The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A high resolution view suitable for qunatitative analysis is available in the library (CIL:38862). Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 9772

• Didinium nasutum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cytoplasm
• Ectosymbiont
• Eukaryotic cell
• Eukaryotic Protist
• Extrusomes

• Carolina Biological Supply Company, NC, U.S.A.
• Cortical cytoskeleton organization
• Cytoplasm organization
• Detection of symbiont
• Plasma membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12299

Doi: https://doi.org/10.6075/J0ZK5FT1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12299

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12