Allen, Richard (2021). CIL:7332, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00R9NF4

High resolution image of the decorated ER tubules and 'rails' on the surface of the ER next to the myonemal sheet. Dense rods accompany the surface ridges adjacent to the myonemes. Pellicular pores are the peritrich homologue to clathrin coated pits. TEM taken on 6/9/69 by R. Allen with Philips 300 operating at 60kV. Neg. 36,500X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis.

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; unprocessed raw data; raw unprocessed image
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3587

• Opercularia
• Opercularia coarctata

• Cell by organism
• Ciliated Protist
• Contractile myonemes
• Eukaryotic cell
• Eukaryotic Protist
• Pellicular pores

• Cambridge Culture Collection, Cambridge, England, # LB1657/1
• Cytoskeleton organization
• Regulation of contractility
• Regulation of liquids
• Trans membrane transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7332

Doi: https://doi.org/10.6075/J00R9NF4

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7332

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12