Wood, Christopher S.; Schmitz, Karl R.; Bessman, Nicholas J.; Setty, Thanuja Gangi; Ferguson, Kathryn M.; Burd, Christopher G. (2021). CIL:13464, Saccharomyces cerevisiae S288c. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0SF2TTH

Kre2-GFP is not efficiently maintained in the Golgi apparatus when Pik1-mediated PtdIns4P synthesis ceases, implicating Pik1 signaling in retrograde trafficking of Golgi residents. In this image, Kre2-GFP is expressed in the pik1-83 mutant strain at permissive temperature of 26C. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4D 26C pik1-83 panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4D include CIL#s 13460, 13461, 13462, 13463, 13464, 13465, 13466, 13467.

• 2021

• Bessman, Nicholas J.
• Burd, Christopher G.
• Ferguson, Kathryn M.
• Schmitz, Karl R.
• Setty, Thanuja Gangi
• Wood, Christopher S.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: constrained iterative deconvolution
Data qualification: Processed;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 6596

• Saccharomyces cerevisiae S288c

• Golgi apparatus
• Integral to membrane
• Trans-Golgi network
• Vacuolar lumen

• 1-phosphatidylinositol 4-kinase activity
• Alpha-1,2-mannosyltransferase activity
• Cell wall mannoprotein biosynthetic process
• Phosphatidylinositol-mediated signaling
• Pik1-83
• Protein glycosylation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13464

Doi: https://doi.org/10.6075/J0SF2TTH

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13464

Reference

• J Cell Biol. 187: 967-975. 2009 https://www.ncbi.nlm.nih.gov/pubmed/?term=20026658

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12