Huranova, M.; Ivani, I.; Benda, A.; Poser, I.; Brody, Y.; Hof, M.; Shav-Tal, Y.; Neugebauer, K.M.; Stanek, D. (2021). CIL:26596, Homo sapiens, cervical epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0765D7P

HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U1-70K and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The U1-70K protein is confined to the nucleoplasm and enriched in splicing factor compartments and Cajal Bodies (bright dots). From Fig 4A in Huranova et al. 2010 J Cell Biol 191:75-86.

• 2021

• Benda, A.
• Brody, Y.
• Hof, M.
• Huranova, M.
• Ivani, I.
• Neugebauer, K.M.
• Poser, I.
• Shav-Tal, Y.
• Stanek, D.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Processed

• Cell Image Library Group ID: 10294

• Homo sapiens

• Cervical epithelium
• Pre-mRNA
• SnRNP
• Spliceosomal complex

• HeLa
• RNA metabolic process
• RNA splicing

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26596

Doi: https://doi.org/10.6075/J0765D7P

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26596

Reference

• M Huranova et al. 2010 J Cell Biol 191:75-86 https://doi.org/10.1083/jcb.201004030

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2022-08-12