Tani, Tomomi; Allen, Richard (2021). CIL:9691, Paramecium multimicronucleatum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0K073FF

Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Measuring membrane tension of the in vitro CV. To measure the build up of tension by the CV membrane an in vitro CV surrounded by a thin layer of cytosol containing ATP was mounted on the tip of a holding pipette and a thin glass rod or sphere, mounted on the tip of a microcantilever, was rested on the CV's membrane. During rounding of the CV the sphere or glass rod was pushed upward which is then recorded as an output voltage of a position sensor. This can be converted into force which, in turn, can be converted into tension. The tension of the rounded CV membrane increased 35-fold over the tension of the relaxed membrane. A DVD resolution version of this is available at CIL:10409.

• 2021

• Allen, Richard
• Tani, Tomomi

Preparation: living tissue
Relation to intact cell: isolated subcellular component
Item type: microscopy; modes of light microscopy
Imaging mode: differential interference contrast microscopy
Parameter imaged: refractive index
Source of contrast: boundaries between regions with different refractive index
Visualization methods: miscellaneous fill
Processing history: film; unprocessed raw data

• Cell Image Library Group ID: 2656

• Paramecium multimicronucleatum

• Ciliated Protist
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist

• Cellular water homeostasis

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 9691

Doi: https://doi.org/10.6075/J0K073FF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9691

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=10607534
• Tani T, Allen RD, Naitoh Y. Periodic tension development in the membrane of the in vitro contractile vacuole of Paramecium multimicronucleatum: modification by bisection, fusion and suction. J Exp Biol. 2000 Jan;203(Pt 2):239-51. https://doi.org/10.1242/jeb.203.2.239

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2022-08-12