Cronshaw, Janet; Matunis, Michael (2021). CIL:40319, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MW2G0J

Transmission electron micrograph of a thin section of the nucleus of a HeLa cell with mutant ALADIN. This grazing section of the nucleus shows en face views of nuclear pores which appear to have a normal ultrastructure. From a study of the role of the nuclear porin ALADIN. Other images in this group show further details of nuclear pore structure in normal and mutant cells.

• 2021

• Cronshaw, Janet
• Matunis, Michael

Normal HeLa cells expressing were fixed with glutaraldehyde, embedded in epoxy resin, thin sectioned, and imaged using a Philips CM10 TEM.
See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; uranyl salt; lead salt
Data qualification: Processed

• Cell Image Library Group ID: 11064

• Homo sapiens

• Cervical carcinoma
• Epithelial cell
• Nuclear envelope
• Nuclear pore

• HeLa
• Nuclear envelope organization
• Nuclear transport
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40319

Doi: https://doi.org/10.6075/J0MW2G0J

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40319

Reference

• Cronshaw, J. M., & Matunis, M. J. (2003). The nuclear pore complex protein ALADIN is mislocalized in triple A syndrome. Proceedings of the National Academy of Sciences of the United States of America, 100(10), 5823–5827. https://doi.org/10.1073/pnas.1031047100
• J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827 https://www.ncbi.nlm.nih.gov/pubmed/?term=12730363

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2022-08-12