Ammar, El-Desouky; Hogenhout, Saskia A. (2021). CIL:12588, Spiroplasma kunkelii, Zea mays, plant cell, prokaryotic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZC81K0

Immuno-gold labeling of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) in sieve elements and other phloem cells of infected maize leaves. CIL:19126, from the same image group, is a labeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Related images from the same contributor are also available in the Library.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.

Detailed methods: Tissue was processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Gold particles used in immunolabeling CSS were 10nm. Negatives were scanned later to generate these electronic files.

Preparation: tissue in epoxy resin embedment; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt; uranyl salt; gold
Data qualification: Processed

• Cell Image Library Group ID: 9974

• Spiroplasma kunkelii
• Zea mays

• Plant cell
• Plant-type cell wall
• Prokaryotic cell

• Pathogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12588

Doi: https://doi.org/10.6075/J0ZC81K0

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12588

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2022-08-12