Hatano, Toshiyuki; Sluder, Greenfield (2021). CIL:25709, Homo sapiens, retinal pigment epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TX3DBS

Time lapse series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using differential interference contrast optics. A movie prepared from the time series is grouped in the Cell Image Library.

• 2021

• Hatano, Toshiyuki
• Sluder, Greenfield

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica HCX PLAN APO 100x NA 1.35 DIC objective lens. images were captured at 30 second intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8516

• Homo sapiens

• Retinal pigment epithelium

• Cell proliferation
• HTERT-RPE1
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25709

Doi: https://doi.org/10.6075/J0TX3DBS

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25709

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12