Ris, Hans (2021). CIL:9984, notopthalmus viridescens, erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05B01KG

Newt (Notopthalmus viridescens erythrocytes were isolated in 0.1M KCl, spread on the surface of Na-citrate, picked up on formvar-carbon films, fixed with glutaraldehyde and paraformaldehyde and negatively stained with 2% sodium tetraborate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 55 degrees. A grouped image of the same field at a 44 degree tilt provides an oblique stereo view of the tangle of chromatin fibers in the dispersed nucleus.

• 2021

• Ris, Hans

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue
Relation to intact cell: spread nucleus
Item type: film
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: other negative stain
Processing history: unprocessed raw data

• Cell Image Library Group ID: 2467

• Notopthalmus viridescens

• Erythrocyte
• Nuclear chromatin

• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9984

Doi: https://doi.org/10.6075/J05B01KG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9984

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=7022124
• Ris H. Stereoscopic electron microscopy of chromosomes. Methods Cell Biol. 1981;22:77-96. https://doi.org/10.1016/s0091-679x(08)61871-3

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2022-08-12