Ammar, El-Desouky; Hogenhout, Saskia A. (2021). CIL:12583, Spiroplasma kunkelii, Dalbulus maidis, fat cell, prokaryotic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N878GB

Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing fat tissue. Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:19121, from the same image group, is a labeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005)
Ann. Entomol. Soc. Am. 98(6): 820Ð826.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; propidium iodide
Data qualification: Processed

• Cell Image Library Group ID: 9985

• Dalbulus maidis
• Spiroplasma kunkelii

• Fat cell
• Nucleus
• Prokaryotic cell

• Pathogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12583

Doi: https://doi.org/10.6075/J0N878GB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12583

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12