Allen, Richard (2021). CIL:36788, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01N7ZRD

The collapsed cytopharyngeal membrane extends from the open cytopharynx into the cytosol. This membrane is associated with many docked acidosomes, vesicles that contain V-type proton translocating ATPases and cause the acidification of the phagosome after they fuse with the phagosome. TEM taken on 11/4/68 by R. Allen with Philips 300 operating at 60kV. Neg.16,000X. Bar = 0.5µm. Published in J. Cell Biol. 64:497-503, 1975. Adapted with permission.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39177) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 10106

• Paramecium caudatum

• Cell by organism
• Ciliated Protist
• Cytopharynx
• Eukaryotic cell
• Eukaryotic Protist
• Oral apparatus
• Vacuolar proton-transporting V-type ATPase, V0 domain

• Digestion
• Digestive system process
• Phagocytosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36788

Doi: https://doi.org/10.6075/J01N7ZRD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36788

Reference

• Allen RD. Evidence for firm linkages between microtubules and membrane-bounded vesicles. J Cell Biol. 1975 Feb;64(2):497-503. https://doi.org/10.1083/jcb.64.2.497

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2022-08-12