CIL:10007, Didinium nasutum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Sage, M.; Allen, Richard (2021). CIL:10007, Didinium nasutum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09Z940Z
- Description
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An image of one of several contractile vacuole pores of the proter of a dividing cell. This is presumably at an early stage of development as division is not yet complete. This section shows the pore to be encased in microtubules. The micrograph shows the microtubules cut transversely on one side so one can count them and obliquely on the other side of the pore. Whether the CV is yet functional at this stage is not known although the pore septum appears as two flaps of membrane. The pore lies close to the division furrow in the proter. TEM taken on 5/21/69 by M. Sage with Philips 300 operating at 60kV. Neg. 14,800X.Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:4666) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: film; Print from negative scanned for Photoshop.
Data qualification: Processed;spatialmeasurements - Series
- Scientific Name
- Anatomy
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 10007
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10007
- Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/
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- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12