Allen, Richard (2021). CIL:36763, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0R78D7T

Cross sections of a pair of basal bodies showing the triplet makeup of the 9 fibers; only one microtubular fiber is complete in each triplet while the other two microtubular fibers share some subunits with their neighbors. TEM taken on 2/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 47,100X. Bar = 0.1µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38899) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 9869

• Paramecium caudatum

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Fiber
• Microtubule basal body

• Microtubule basal body organization
• Microtubule cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36763

Doi: https://doi.org/10.6075/J0R78D7T

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36763

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2022-08-12