Routh, Andrew; Sandin, Sara; Rhodes, Daniela (2021). CIL:39741. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JD4VK1

Images of negatively stained chromatin fibers prepared from tandem arrays of a nucleosome positioning sequence reconstituted with purified histones. These samples contained 80 repeats of a 167 bp DNA sequence reconstituted with core histones alone (leftmost image) plus different amounts of linker histone H5 (33%, 67%, and 100% respectively).
See: Fig 3b in Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877.

• 2021

• Rhodes, Daniela
• Routh, Andrew
• Sandin, Sara

Reconstituted chromatin was fixed with 0.1% glutaraldehyde for 30 min, deposited on glow discharged carbon films, negatively stained with aqueous uranyl acetate, and examined with a Philips 2085 TEM operated at 80 KV.
See also: Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877.

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: negative staining; uranyl salt
Processing history: montage
Data qualification: Processed

• Cell Image Library Group ID: 10708

• Chromatin
• Nuclear chromatin

• Cellular component organization or biogenesis
• Chromatin organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39741

Doi: https://doi.org/10.6075/J0JD4VK1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39741

Reference

• A Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877 https://www.ncbi.nlm.nih.gov/pubmed/?term=18583476

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2022-08-12