Choi, Colin K.; Horwitz, Alan Rick (2021). CIL:9530, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NS0SKD

Adhesion dynamics in a migrating CHO K1 cell. CHO K1 cells expressing paxillin-mEGFP were plated onto coverslips that were preincubated with a solution of 5 ug/ml fibronectin overnight at 4o C. The cells were visualized at 60x (1.45NA) with an Olympus IX70 inverted microscope using Total Internal Reflection Optics (through the objective mode) equipped with a Qimaging Retiga Exi CCD camera (0.1073 microns/pixel). Images were acquired every 5 seconds for a total of 15 minutes. Note the formation of small, nascent adhesions near the leading edge of protrusions. These adhesions form (in the lamellipodium) and their formation is coupled to actin polymerization. Many adhesions turnover (at the lamellipodium/lamellum interface), as the protrusion proceeds. Some adhesions also elongate and grow (mature), particularly during pauses in protrusion. The fraction of adhesions that elongate, and the rate at which they do so, is cell type dependent. Note also, the sliding and disassembly of adhesions in retracting regions of the cell. The movie was taken by Colin Choi in the Rick Horwitz laboratory (University of Virginia).

• 2021

• Choi, Colin K.
• Horwitz, Alan Rick

Preparation: dispersed cells in vitro
Relation to intact cell: unfixed tissue
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cricetulus griseus

• Cell-substrate adherens junction
• Epithelial cell
• Focal adhesion

• CHO-K1
• Focal adhesion assembly
• Negative regulation of cell adhesion involved in substrate-bound cell migration

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 9530

Doi: https://doi.org/10.6075/J0NS0SKD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9530

Reference

• PubMed article: <@ https://www.ncbi.nlm.nih.gov/pubmed/?term=20729930
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=19160484
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=19160484
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=20729930

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12