Allen, Richard (2021). CIL:12107, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TH8KVB

A tangential view through a dikinetid shows two microtubular ribbons extending toward the pellicle, one from each basal body, and how they overlap with ribbons from other basal bodies under the pellicle. The peroxisome has one membrane but is filled with numerous tiny circular profiles that may represent tiny tubules. These tubules are much smaller than the membranous cristae in the mitochondria and are more probably proteinaceous in composition. TEM taken on 6/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2862) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3321

• Colpoda cucullus

• Axoneme
• Cartwheel complex
• Cell by organism
• Cell cortex
• Ciliated Protist
• Cilium
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Peroxisome

• Cambridge Culture Collection, Cambridge, England # LB1615/1
• Cell motility
• Cortical cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12107

Doi: https://doi.org/10.6075/J0TH8KVB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12107

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12