Hartman, Tiffiney R.; Zinshteyn, Daniel; Schofield, Heather K.; Nicolas, Emmanuelle; Okada, Ami; O'Reilly, Alana M. (2021). CIL:13741, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CJ8C73

Boi (red) localizes predominantly to apical cells in Drosophila wild-type germaria but not in germaria with UAS-boi[RNAi] expression (CIL#13742). Germ cells are identified using anti-Vasa (blue). Image is Fig 2D in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).

• 2021

• Hartman, Tiffiney R.
• Nicolas, Emmanuelle
• O'Reilly, Alana M.
• Okada, Ami
• Schofield, Heather K.
• Zinshteyn, Daniel

Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). Primary antibodies were 1:50 rat-antiBoi (Hartman et al. 2010) 1:2,000 rabbit anti-Vasa (Hay et al., 1990); Secondary antibodies used were Cy3 and Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; Cy3; Cy5
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8382

• Drosophila melanogaster

• Follicle cell
• Follicle stem cell
• Germ line cell
• Germ line stem cell
• P granule

• ATP-dependent helicase activity
• Cell adhesion
• Germ-line cyst formation
• Germarium-derived egg chamber formation
• MRNA 3'-UTR binding
• Positive regulation of translation
• Protein binding
• RNA binding
• Smoothened signaling pathway

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13741

Doi: https://doi.org/10.6075/J0CJ8C73

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13741

Reference

• J Cell Biol. 2010. 191: 943-952. https://www.ncbi.nlm.nih.gov/pubmed/?term=21098113

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12