Farquhar, Marilyn G.; Palade, George E. (2021). CIL:7620, Rattus rattus, epithelial cell, intestinal epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GH9GNP

Junctional complex between two cells in the epithelium of the rat intestinal mucosa. The tight junction (also known as zonula occludens) is the structure located nearest to the lumen. The elimination of the intercellular gap (approx 90 angstroms) is clearly visible, but the fusion line of the two apposed membranes cannot be clearly distinguished at this magnification. The adherens junction (also known as zonula adherens) is the next structure. A relatively wide intercellular space (approx 200 angstroms) is maintained throughout this junction. Extensive actin filament networks are seen along both sides of the junction which are continuous with the terminal web into which the filamentous rootlets of the mircovilli penetrate. Dense material can be seen along parts of both sides of the junction. The limits of a desmosome are marked by the edges of the dense plaques. Desmosomes are characterized by a wide intercellular space (approx 240 angstroms) bisected by an intermediate line. Bundles of intermediate filaments (diameter approx 80 angstroms) converge into a dense plaque on each side of the desmosome. The trilaminar structure of the cell membrane is clearly seen along the microvilli, between the Zonula Adherens and the desmosome and within the desmosome.First description of 1) tight junctions and 2) the junctional complexes between epithelial cells, consisting of tight junction, adherens junction and a desmosome. Identical labeled image available as CIL# 7621. Image published as Figure 1 in Farquhar and Palade, 1963.

• 2021

• Farquhar, Marilyn G.
• Palade, George E.

Fixative was injected into the intestinal lumen of a rat (3% osmium tetroxide buffered at pH 7.6 with acetate- veronal). Tissue may have also been fixed in potassium permanganate. Tissue blocks were embedded in Epon. Sections were stained with lead hydroxide. Micrograph was taken at original magnification of 96,000 X with a Siemens Elmiskop I, operating at 60 or 80 kv, with a double condenser, and a 50 µ objective aperture. Digitization process: conversion from a 1200 dpi tiff, 3820 x 3342 pixels. Original image created on January 5, 1962. Original resource provided as a 3.25 x 4 inch lantern slide. Research conducted at The Rockefeller Institute of Medical Research (New York, NY).

Preparation: osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: lead salt
Processing history: digitized lantern slide
Data qualification: Processed;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 10228

• Rattus rattus

• Actin filament
• Brush border membrane
• Desmosome
• Epithelial cell
• Intermediate filament
• Intestinal epithelial cell
• Microvillus
• Occluding junction
• Plasma membrane
• Zonula adherens

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7620

Doi: https://doi.org/10.6075/J0GH9GNP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7620

Reference

• J Cell Biol 17: 375-412. 1963. https://www.ncbi.nlm.nih.gov/pubmed/?term=13944428
• Marilyn G. Farquhar, George E. Palade; Junctional complexes in various epithelia. J Cell Biol 1 May 1963; 17 (2): 375–412. https://doi.org/10.1083/jcb.17.2.375

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2022-08-12