CIL:40263, Mus musculus, pyramidal cell, stellate cell, astrocyte of the cerebral cortex
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- Collection
- Cite This Work
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Reid, R. Clay (2021). CIL:40263, Mus musculus, pyramidal cell, stellate cell, astrocyte of the cerebral cortex. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0V9886B
- Description
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Serial electron micrograph series through layers 1-4 of the primary visual cortex of the adult mouse. Reconstruction encompasses 450 X 350 X 52 um volume of tissue and contains ~ 1500 cell bodies. This same area of tissue was functionally characterized for orientation and direction selectivity using two photon calcium imaging. The reconstruction is also available at:
http://openconnectomeproject.org/Bock11/
For more information see: Bock DD, Lee WC, Kerlin AM, Andermann ML, Hood G, Wetzel AW, Yurgenson S, Soucy ER, Kim HS, Reid RC. Network anatomy and in vivo physiology of visual cortical neurons. Nature. 2011 Mar 10;471(7337):177-82. PMID: 21390124. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. - Date Issued
- 2021
- Researcher
- Methods
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The animal was perfused transcardially (2% paraformaldehyde/2.5% glutaraldehyde). Seven 300 um thick coronal vibratome sections were cut. The vibratome sections were post-fixed and stained with 1% osmium tetroxide/1.5% potassium ferrocyanide followed by 1% uranyl acetate, dehydrated with a graded ethanol series, and embedded in resin (TAAB 812 Epon, Canemco). Serial thin (<50 nm) sections were cut on a Leica U6 ultramicrotome, picked up on pioloform-coated slot grids, and then post-stained with uranyl acetate and lead citrate. Serial sections were imaged with a JEOL 1200EX, magnification, 2000.0 X, accelerating voltage, 120.0 kv, and compiled into a composite image.
- Technical Details
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Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Visualization methods: uranyl salt
Data qualification: Processed - Scientific Name
- Anatomy
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40263
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40263
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0Z31ZFZ
Source data
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- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12