Faust, James J.; Capco, David G. (2021). CIL:40673, Mesocricetus auratus, early embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MG7NVK

Stereo pair of an embedment-free section of a detergent-extracted 8-cell embryo reveals the cytoskeletal interior in greater detail than is afforded in resin-embedded sections. The specimen is viewed at an angle by selecting a stereo pair of -12 and 6 degrees (left to right), consequently the bottom left corner appears lower in the stereomicrograph. At later stages of development the specialized intermediate filament network in mammalian eggs and embryos (i.e., the cytoskeletal whorls) become parallel bundles as they are preparing to disassemble into individual intermediate filaments. Scale bar is 1.0 µm.

• 2021

• Capco, David G.
• Faust, James J.

Sections were imaged with a Phillips CM300 transmission electron microscope. See Capco DG, McGaughey RW. Dev Biol. 1986 Jun;115(2):446-58 for details on specimen processing.

Preparation: detergent permeabilized; chemically fixed tissue; osmium tetroxide fixed tissue; embedment medium removal
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: detection of electrons
Source of contrast: differences in adsorption or binding of stain
Processing history: stereo pair

• Cell Image Library Group ID: 11808

• Mesocricetus auratus

• Early embryonic cell
• Intermediate filament cytoskeleton

• Intermediate filament cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40673

Doi: https://doi.org/10.6075/J0MG7NVK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40673

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2022-08-12