Braine, Catherine; Rice, Marian (2021). CIL:34562, Drosophila melanogaster, muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VT1S3Q

Drosophila melanogaster thoracic tergotrochanter muscle from a four week-old wild-type animal. A longitudinal section of myocyte is displayed. Sections of parallel myofibrils are seen with distinguishable sarcoplasmic reticulum.

• 2021

• Braine, Catherine
• Rice, Marian

Tissue was dissected using a freeze-fracture technique, fixed in 2.5% glutaraldehyde in 0.1 M sodium cacadylate buffer, pH 7.4, post-fixed in 2% osmium tetroxide solution, stained in 1% uranyl acetate, and embeddeded in EMbed 812 resin. Sections were post-stained with 2% uranyl acetate and stained with Reynolds's Lead Citrate. Images were obtained using transmission electron microscopy techniques, employing a Phillips CM-100 transmission electron microscope. Images were taken on Kodak EM 4489 film, scanned with a Microtek ScanMaker i900, and post-processed using Adobe Photoshop CS4. This image was taken at a magnification of 8900x.

Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; uranyl salt; lead salt
Processing history: digitized film
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 8583

• Drosophila melanogaster

• Muscle cell
• Myofibril
• Sarcoplasmic reticulum

• Flight

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34562

Doi: https://doi.org/10.6075/J0VT1S3Q

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34562

Creative Commons Attribution 4.0 International Public License

• UC Regents

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2022-08-12