Vale, Ronald D.; Spudich, James A.; Griffis, Eric R. (2021). CIL:11991, Drosophila melanogaster, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05H7F1N

TIRF time-lapse imaging of EB1-GFP (green) and mCherry-tubulin (red). EB1-GFP is seen on microtubule tips that grow to the cortex but does not accumulate on stable microtubule bundles at the equator, in contrast to kinesin-6 . Corresponds to video 8 from J Cell Bio 186:727-738, 2009.
Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http://www.micro-manager.org).

• 2021

• Griffis, Eric R.
• Spudich, James A.
• Vale, Ronald D.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: microtubule polymerization; mCherryFP
Processing history: unprocessed raw data; color combine
Data qualification: Raw

• Cell Image Library Group ID: 3733

• Drosophila melanogaster

• EB1
• Epithelial cell
• Microtubule

• Microtubule polymerization
• S2

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11991

Doi: https://doi.org/10.6075/J05H7F1N

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11991

Reference

• J Cell Bio 186:727-738, 2009 https://www.ncbi.nlm.nih.gov/pubmed/?term=19720876
• Vale RD, Spudich JA, Griffis ER. Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. J Cell Biol. 2009 Sep 7;186(5):727-38. https://doi.org/10.1083/jcb.200902083

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12