Goodenough, Ursula W.; Staehelin, L. Andrew (2021). CIL:38704, Chlamydomonas reinhardtii. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08W3BZQ

Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. These thylakoid membranes from this mixotropically grown ac-5 mutant show the particle size and distribution typical of unstacked membranes. See Fig 16 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

• 2021

• Goodenough, Ursula W.
• Staehelin, L. Andrew

Membranes were prepared, cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Preparation: cryofixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed

• Cell Image Library Group ID: 11387

• Chlamydomonas reinhardtii

• Chloroplast
• Plastid
• Plastid thylakoid membrane

• Ac-31
• Ac-5
• Organelle organization
• Photosynthesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 38704

Doi: https://doi.org/10.6075/J08W3BZQ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38704

Reference

• Goodenough UW, Staehelin LA. Structural differentiation of stacked and unstacked chloroplast membranes. Freeze-etch electron microscopy of wild-type and mutant strains of Chlamydomonas. J Cell Biol. 1971 Mar;48(3):594-619. https://doi.org/10.1083/jcb.48.3.594
• UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619. https://www.ncbi.nlm.nih.gov/pubmed/?term=4396088

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2022-08-12