Hotta, Azusa; Kawakatsu, Tomomi; Nakatani, Tomoya; Sato, Toshitaka; Matsui, Chiyuki; Sukezane, Taiko; Akagi, Tsuyoshi; Hamaji, Tomoki; Grigoriev, Ilya; Akhmanova, Anna; Takai, Yoshimi; Mimori-Kiyosue, Yuko (2021). CIL:12288, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N0158C

To determine the extent to which extracellular matrix proteins colocalize with the microtubule-anchoring factor LL5s (LL5α), MCF-10A epithelial cells were immunostained with antibodies to the secreted autocrine laminin-5 (green), the laminin receptor integrin α3 (blue) and LL5s (red). Analyses suggest that LL5s are colocalized with activated pools of integrins associated with laminin-5. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 3 Panel D-a from J Cell Biol (2010) 189 (5):901-917.

• 2021

• Akagi, Tsuyoshi
• Akhmanova, Anna
• Grigoriev, Ilya
• Hamaji, Tomoki
• Hotta, Azusa
• Kawakatsu, Tomomi
• Matsui, Chiyuki
• Mimori-Kiyosue, Yuko
• Nakatani, Tomoya
• Sato, Toshitaka
• Sukezane, Taiko
• Takai, Yoshimi

MCF-10A epithelial cells were fixed with 1:1 mixture of methanol/acetone (-20° C, 10 min), permeabilized with 0.5% Triton in PBS, and immunostained sequentially with primary, then secondary antibodies and (Cy5, 633 nm laser), laminin-5 (Cy2, 488 nm laser) and integrin a3 (rhodamine red-X, 543 nm laser), and observed using a confocal laser-scanning microscope system. For additional details see: J. Cell Biol. 2010. 189(5):901-917

Preparation: methanol fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Cy2; Cy5; X-Rhodamine
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 8194

• Homo sapiens

• Cell cortex part
• Epithelial cell
• Microtubule

• Cell-substrate adhesion
• Epithelial cell migration
• Establishment or maintenance of apical/basal cell polarity
• MCF 10A

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12288

Doi: https://doi.org/10.6075/J0N0158C

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12288

Reference

• Hotta A, Kawakatsu T, Nakatani T, Sato T, Matsui C, Sukezane T, Akagi T, Hamaji T, Grigoriev I, Akhmanova A, Takai Y, Mimori-Kiyosue Y. Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5alpha/beta. J Cell Biol. 2010 May 31;189(5):901-17. https://doi.org/10.1083/jcb.200910095
• J. Cell Biol. 2010. 189(5):901-917 https://www.ncbi.nlm.nih.gov/pubmed/?term=0513769

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2025-01-30