Asensio, Cédric S.; Sirkis, Daniel W.; Edwards, Robert H. (2021). CIL:13763, Rattus norvegicus, pheochromocytoma, adrenal gland cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W094MN

Electron micrograph of PC12 cells transfected with AP-3 siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM AP-3delta1 siRNA and fixed with 2.5% glutaraldehyde in calcium-/magnesium-free PBS. The discs were washed three times with 0.1 M sodium cacodylate buffer, pH 7.2, and postfixed for 30 min on ice with 1% osmium tetroxide in cacodylate buffer containing 1.6% potassium ferricyanide. The discs were then washed three times with cacodylate buffer, three times with water, incubated with 0.5% uranyl acetate for 30 min (in the dark), and washed again with water. The samples were dehydrated in a graded series of ethanols while progressively lowering the temperature from 4°C to −40°C then embedded in epon resin. After peeling off the aclar, 60-nm sections were cut and viewed in an electron microscope (FEI Tecnai 12; Phillips) at 120 kV, images were captured with a digital camera at 6,800 magnification. Image is similar to Fig 6C right panel (CIL# 13764) of J Cell Biol. 2010. 191: 1173-1187.

• 2021

• Asensio, Cédric S.
• Edwards, Robert H.
• Sirkis, Daniel W.

Preparation: tissue in epoxy resin embedment; glutaraldehyde fixed tissue
Relation to intact cell: cryostat-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; uranyl salt
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 7925

• Rattus norvegicus

• Adrenal gland cell
• AP-3 adaptor complex
• Mitochondrion
• Pheochromocytoma
• Plasma membrane
• Trans-Golgi network transport vesicle
• Transport vesicle

• PC-12
• Regulated secretory pathway
• Vesicle-mediated transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13763

Doi: https://doi.org/10.6075/J0W094MN

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13763

Reference

• J Cell Biol. 2010. 191: 1173-1187. https://www.ncbi.nlm.nih.gov/pubmed/?term=21149569

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12