Caro, Lucien (2021). CIL:37119, Cavia porcellus, pancreas cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZP44TZ

Electron microscope autoradiograph showing the high concentration of radioactivity over the condensing vacuoles of the Golgi complex twenty minutes after injection. Image corresponds to Figure 7 in J. Cell Biol. 20:473-495. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

• 1964-06-04

• 2021

• Caro, Lucien

Anesthetized animals were intravenously injected with 1-5 millicuris of DL-leucine 4,5-H^3 with a specific activity of 3570 mc/mM. At times ranging from 4 min to 15 hrs after injection the pancreas was removed and fixed in osmium tetroxide. Tissue was embedded in methacrylate, sectioned, and post-stained with uranyl acetate.

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x30,000.

Preparation: osmium tetroxide fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); electron microscope autoradiograph
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; metabolically incorporated radioisotope
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10844

• Cavia porcellus

• Golgi-associated vesicle
• Pancreas cell
• Vacuole

• Protein secretion
• Protein transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 37119

Doi: https://doi.org/10.6075/J0ZP44TZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37119

Reference

• J Cell Biol. 1964 Mar;20:473-95. https://www.ncbi.nlm.nih.gov/pubmed/?term=14128049

Other resource

• George E. Palade EM Slide Collection: http://cushing.med.yale.edu/gsdl/cgi-bin/library?c=palade&a=d&d=DpaladeARC

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2023-08-17